Demonstration of the causal agent is the diagnostic procedure in acute Chagas disease. It almost always yields positive results, and can be achieved by:
Microscopic examination: a) of fresh anticoagulated blood, or its buffy coat, for motile parasites; and b) of thin and thick blood smears stained with Giemsa, for visualization of parasites.
Isolation of the agent: a) inoculation in culture with specialized media (e.g. NNN, LIT); b) inoculation into mice; and c) xenodiagnosis, where uninfected triatomine bugs are fed on the patient’s blood, and their gut contents examined for parasites 4 weeks later.
For more information view the source: Center for Disease Control
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